Imod ctfphaseflip
WitrynaStripe-based CTF correction (with ctfphaseflip from Imod) Creation of tomograms with Dynamo; These preprocessing operations that go from the tilt series to the tomograms can be used for any sample. Tomogram management. This part is specific to the particular geometry of the proteins of interest. Cataloguing the tomograms (archiving) Witryna30 mar 2024 · CTF correction: CTF correction of a set of input tilt-series using the IMOD procedure. More info: ctfphaseflip doc; Dose filter: Tilt-series dose filtering based on …
Imod ctfphaseflip
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WitrynaIn this GUI, CTF estimation and correction are done using CTFFIND4 and IMOD's ctfphaseflip. This part will not be used in the workshop. Reconstruction Binned reconstruction. Click on binned reconstruction to create a binned tomogram using the previously created binned aligned tilt series. Adapt the tomogram height if necessary … Witryna19 maj 2024 · CTF correction: CTF correction of a set of input tilt-series using the IMOD procedure. More info: ctfphaseflip doc; Dose filter: Tilt-series dose filtering based on …
Witryna25 wrz 2024 · Defocus estimation was performed using IMOD ctfplotter , phase flipping was performed using NovaCTF or ctfphaseflip, and dose weighting was performed using IMOD mtffilter. Tomograms were aligned using IMOD etomo and subsequently reconstructed using NovaCTF, with the exception of eight tomograms that were used … WitrynaStructure, Volume 23 Supplemental Information Cryoelectron Tomography of the NAIP5/NLRC4 Inflammasome: Implications for NLR Activation Christoph A. Diebolder, Els F. Halff, Abraham J. Koster, Eric G. Huizinga, and Roman I.
Witryna4 lut 2024 · The CTF was estimated using GCTF 61 and then the IMOD ctfphaseflip implementation was used for phase correction 62. Tomograms were manually segmented using AMIRA (Thermo Fisher Scientific) or ... Witryna4/2/10: Fixed initialization problem in Ctfphaseflip's detection of bad view numbers and fixed the warning when a simple one-line file is used. 4/1/10: Fixed bug in Ctfplotter …
Witryna-help (-he) OR -usage Print help output -StandardInput Read parameter entries from standard input AUTHORS Quanren Xiong David Mastronarde John Heumann SEE …
WitrynaChanges for IMOD 4.11. Changes marked with ** are particularly noteworthy, ones marked with * may also be of interest to typical users. ... * 10/22/18: Ctfphaseflip is … porto campus onlineWitrynaAuthors: Federico P. de Isidro Gomez ([email protected]) [1] # * # * [1] Centro Nacional de Biotecnologia, CSIC, Spain # * # * This program is free software; you can redistribute it and/or modify # * it under the terms of the GNU General Public License as published by # * the Free Software Foundation; either version 2 of the License, or # * … porto brief nach schottlandporto brief nach ungarnWitryna1 lip 2016 · Tilt series alignment and tomogram reconstruction were done using the batch reconstruction protocol of etomo in IMOD software (version 4.12.10; [56, 57] ). … porto buchhandlungWitrynaimod.protocols.protocol_ctfCorrecion module¶ class imod.protocols.protocol_ctfCorrecion.ProtImodCtfCorrection (**kwargs) [source] ¶. Bases: pwem.protocols.protocol ... porto brief post modern 2023WitrynaThe CTF was estimated using GCTF 61 and then the IMOD ctfphaseflip implementation was used for phase correction 62 . B Nearest neighbor distance distribution of S protein densities on the platelet ... porto brief nach thailandWitrynaExposure-filtered images were then used for tomogram reconstruction in the IMOD software package (39). CTF-correction was performed by the “ctfphaseflip”-program implemented in IMOD (40). CTF-corrected unbinned tomograms were reconstructed and VLPs and virus particles were identified in the 8x-binned tomograms using the Amira optipak glasses case